By S. Morse
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5. A) RS of 80 mg DPA in 1 mL 1N KOH in a glass capillary. B) SERS of 1 mg DPA in 1 mL water in a silver-doped sol-gel filled glass capillary. Spectral conditions: A) 450 mW of 785 nm, 5 min acquisition time and B) 150 mW of 785 nm, 1-minute acquisition time; both 8 cm-1 resolution. Next the pH dependence of both the measurement and analyte was considered. This could be significant if an acid or a base is used to digest spores and extract the CaDPA. , 1988), which in turn affects the plasmon field and the Raman signal enhancement.
83 (inset). Overall there is only a modest decrease in intensity for most of the peaks as a function of pH. For example, the 1006 cm-1 peak assigned to the pyridine ring stretching mode decreases by ~7% from pH 2 to 11. 5. These peaks change intensity as pairs. The 795 cm-1 peak loses intensity as the pH becomes basic, while the 812 cm-1 peak gains a little intensity. Similarly, the 1567 cm-1 peak loses intensity as the pH becomes basic, while the 1590 cm-1 peak gains intensity. The intensities of the former pair are plotted as a function of pH in Fig.
There is some similarity in structure in that Groups 1, 2, and 5 have a disulfide bond while Group 3 and Group 4 (TSST-1) do not. , 2010). Some bind zinc in the concave β sheet of the C terminal domain while others bind zinc in a cleft between the two domains. , 2001; Sundstrom, et al, 1996). 2 Genetic analysis of SE genes Analysis of SE genes indicates divergence from a common ancestry. , 2002). In 2001, a cluster of genes with homologies to SE genes was identified and named the enterotoxin gene cluster (egc).